Carbamycholine prevents giant granule-formation in cultured fibroblasts from beige (Chediak-Higashi) mice
نویسندگان
چکیده
Primary embryonic fibroblasts isolated from beige (Chediak-Higashi) mice develop pathognomonic giant granules in vitro. Inclusion in the culture medium of carbamylcholine (carbachol) or phorbol myristate acetate (PMA) results in the generation of morphologically normally granules. Chediak-Higashi fibroblasts are highly susceptible to shape changes induced by colchicine. This abnormal property is also corrected by carbachol and PMA.
منابع مشابه
Correction of characteristic abnormalities of microtubule function and granule morphology in Chediak-Higashi syndrome with cholinergic agonists.
Chediak-Higashi (CH) syndrome is a genetic disorder of children and certain animal species including the beige mouse. We have previously described a membrane abnormality in CH mouse polymorphonuclear leukocytes (PMH). Whereas normal mouse PMN do not form surface caps with concanavalin A except after treatment with agents such as colchicine that inhibit microtubule assembly, CH mouse PMN show sp...
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متن کاملThe giant organelles in beige and Chediak-Higashi fibroblasts are derived from late endosomes and mature lysosomes
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 69 شماره
صفحات -
تاریخ انتشار 1976